mitophagy detection kit (Dojindo Labs)

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Dojindo Labs mitophagy detection kit

MSC-mt internalization promotes <t>mitophagy</t> activation under oxidative stress (A-B) Flow cytometric analysis of mitophagy in L929 cells co-cultured with fluorescently labeled MSC-mt under H 2 O 2 -induced oxidative stress. Mitophagy levels are shown for total cells as well as stratified mt transfer + and mt transfer − subpopulations, showing preferential mitophagy activation in mt transfer + cells. (C-D) Western blot analysis of mitophagy- and survival-related signaling proteins in flow-sorted mt transfer + and mt transfer − L929 cells following co-culture with fluorescently labeled MSC-mt under oxidative stress. Blots show phosphorylated PINK1 (S228), total PINK1, Parkin, total p62, phosphorylated p62 (S349 and S403), pAKT, OXPHOS components, and TOM20, highlighting enhanced PINK1–Parkin signaling and mitophagy-associated p62 processing in mt transfer + cells. (E) Flow cytometric assessment of mitophagy in total, mt transfer + , and mt transfer − populations following co-culture with PINK1-deficient MSC-derived mitochondria (siPINK1-mt) under oxidative stress, showing attenuated mitophagy activation compared with control MSC-mt. (F) Representative immunofluorescence images of L929 cells under control, H 2 O 2 , and H 2 O 2 + MSC-mt conditions, showing depolarized mitochondria (mitoPeDPP, green) and mitophagy signals (mitophagy, red), indicating increased mitophagic engagement under oxidative stress with MSC-mt transfer. Scale bar = 20 μm. (G–J) Flow cytometric analysis of depolarized mitochondria (mitoPeDPP) and mitophagy in L929 cells under H 2 O 2 stimulation with or without fluorescently labeled MSC-mt co-culture. (G) Representative flow cytometry plots. (H) Quantification of the proportions of mitoPeDPP + , mitophagy + , and double-positive cell populations. (I) Mean fluorescence intensity (MFI) of mitophagy signals, with stratification by mt transfer + and mt transfer − populations. (J) MFI of mitoPeDPP signals, with stratification by mt transfer + and mt transfer − populations. All experiments were independently repeated three times (n = 3) and representative images are shown. Data are presented as mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ns, not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Mitophagy Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitophagy+detection+kit/Mitophagy+Detection+Kit/pmc13049664-157-7-10
Average 96 stars, based on 294 article reviews

mitophagy detection kit - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake"

Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake

Journal: Materials Today Bio

doi: 10.1016/j.mtbio.2026.103023

MSC-mt internalization promotes mitophagy activation under oxidative stress (A-B) Flow cytometric analysis of mitophagy in L929 cells co-cultured with fluorescently labeled MSC-mt under H 2 O 2 -induced oxidative stress. Mitophagy levels are shown for total cells as well as stratified mt transfer + and mt transfer − subpopulations, showing preferential mitophagy activation in mt transfer + cells. (C-D) Western blot analysis of mitophagy- and survival-related signaling proteins in flow-sorted mt transfer + and mt transfer − L929 cells following co-culture with fluorescently labeled MSC-mt under oxidative stress. Blots show phosphorylated PINK1 (S228), total PINK1, Parkin, total p62, phosphorylated p62 (S349 and S403), pAKT, OXPHOS components, and TOM20, highlighting enhanced PINK1–Parkin signaling and mitophagy-associated p62 processing in mt transfer + cells. (E) Flow cytometric assessment of mitophagy in total, mt transfer + , and mt transfer − populations following co-culture with PINK1-deficient MSC-derived mitochondria (siPINK1-mt) under oxidative stress, showing attenuated mitophagy activation compared with control MSC-mt. (F) Representative immunofluorescence images of L929 cells under control, H 2 O 2 , and H 2 O 2 + MSC-mt conditions, showing depolarized mitochondria (mitoPeDPP, green) and mitophagy signals (mitophagy, red), indicating increased mitophagic engagement under oxidative stress with MSC-mt transfer. Scale bar = 20 μm. (G–J) Flow cytometric analysis of depolarized mitochondria (mitoPeDPP) and mitophagy in L929 cells under H 2 O 2 stimulation with or without fluorescently labeled MSC-mt co-culture. (G) Representative flow cytometry plots. (H) Quantification of the proportions of mitoPeDPP + , mitophagy + , and double-positive cell populations. (I) Mean fluorescence intensity (MFI) of mitophagy signals, with stratification by mt transfer + and mt transfer − populations. (J) MFI of mitoPeDPP signals, with stratification by mt transfer + and mt transfer − populations. All experiments were independently repeated three times (n = 3) and representative images are shown. Data are presented as mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ns, not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Figure Legend Snippet: MSC-mt internalization promotes mitophagy activation under oxidative stress (A-B) Flow cytometric analysis of mitophagy in L929 cells co-cultured with fluorescently labeled MSC-mt under H 2 O 2 -induced oxidative stress. Mitophagy levels are shown for total cells as well as stratified mt transfer + and mt transfer − subpopulations, showing preferential mitophagy activation in mt transfer + cells. (C-D) Western blot analysis of mitophagy- and survival-related signaling proteins in flow-sorted mt transfer + and mt transfer − L929 cells following co-culture with fluorescently labeled MSC-mt under oxidative stress. Blots show phosphorylated PINK1 (S228), total PINK1, Parkin, total p62, phosphorylated p62 (S349 and S403), pAKT, OXPHOS components, and TOM20, highlighting enhanced PINK1–Parkin signaling and mitophagy-associated p62 processing in mt transfer + cells. (E) Flow cytometric assessment of mitophagy in total, mt transfer + , and mt transfer − populations following co-culture with PINK1-deficient MSC-derived mitochondria (siPINK1-mt) under oxidative stress, showing attenuated mitophagy activation compared with control MSC-mt. (F) Representative immunofluorescence images of L929 cells under control, H 2 O 2 , and H 2 O 2 + MSC-mt conditions, showing depolarized mitochondria (mitoPeDPP, green) and mitophagy signals (mitophagy, red), indicating increased mitophagic engagement under oxidative stress with MSC-mt transfer. Scale bar = 20 μm. (G–J) Flow cytometric analysis of depolarized mitochondria (mitoPeDPP) and mitophagy in L929 cells under H 2 O 2 stimulation with or without fluorescently labeled MSC-mt co-culture. (G) Representative flow cytometry plots. (H) Quantification of the proportions of mitoPeDPP + , mitophagy + , and double-positive cell populations. (I) Mean fluorescence intensity (MFI) of mitophagy signals, with stratification by mt transfer + and mt transfer − populations. (J) MFI of mitoPeDPP signals, with stratification by mt transfer + and mt transfer − populations. All experiments were independently repeated three times (n = 3) and representative images are shown. Data are presented as mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ns, not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Techniques Used: Activation Assay, Cell Culture, Labeling, Western Blot, Co-Culture Assay, Derivative Assay, Control, Immunofluorescence, Flow Cytometry, Fluorescence

Labeling:

Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
Article Snippet: .. Following co-culture, mitophagy was detected using a mitophagy detection kit (Dojindo, Cat# MD01), while depolarized mitochondria were simultaneously labeled with MitoPeDPP (Dojindo, Cat# M466), according to the manufacturers’ instructions. ..

Activity Assay:

Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
Article Snippet: Intracellular reactive oxygen species (ROS) were detected using the DCFH-DA fluorescent probe (Beyotime, Cat# S0033S). .. Mitophagy activity was analyzed using the Mitophagy Detection Kit (Dojindo, Cat# MD01) following the manufacturer's protocol. .. In selected experiments, mitophagy was analyzed in parallel with mitochondrial depolarization using a dual-staining strategy combining the Mitophagy Detection Kit with the mitochondrial depolarization probe mitoPeDPP (Dojindo, Cat# M466), allowing simultaneous evaluation of mitophagy activation and damaged mitochondria at the single-cell level.

Activation Assay:

Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
Article Snippet: Mitophagy activity was analyzed using the Mitophagy Detection Kit (Dojindo, Cat# MD01) following the manufacturer's protocol. .. In selected experiments, mitophagy was analyzed in parallel with mitochondrial depolarization using a dual-staining strategy combining the Mitophagy Detection Kit with the mitochondrial depolarization probe mitoPeDPP (Dojindo, Cat# M466), allowing simultaneous evaluation of mitophagy activation and damaged mitochondria at the single-cell level. ..

Single Cell:

Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
Article Snippet: Mitophagy activity was analyzed using the Mitophagy Detection Kit (Dojindo, Cat# MD01) following the manufacturer's protocol. .. In selected experiments, mitophagy was analyzed in parallel with mitochondrial depolarization using a dual-staining strategy combining the Mitophagy Detection Kit with the mitochondrial depolarization probe mitoPeDPP (Dojindo, Cat# M466), allowing simultaneous evaluation of mitophagy activation and damaged mitochondria at the single-cell level. ..

Membrane:

Article Title: Significance of mitophagy in reactive oxygen species-dependent neuronal apoptosis triggered by pyrrolidinophenones.
Article Snippet: Pyrrolidinophenones (PPs), a class of synthetic cathinones, have emerged as hazardous new psychoactive substances due to their high lipophilicity and potent neurotoxicity.. However, the mechanisms underlying PPsinduced neuronal damage, particularly the roles of mitochondrial reactive oxygen species (ROS) and mitophagy, remain unclear.. In this study, we investigated the interplay among ROS overproduction, mitochondrial dysfunction, mitophagy, and apoptosis in human neuronal cells exposed to representative PPs.

Injection:

Article Title: Steroids and flavonoids in TRQ ameliorates chronic obstructive pulmonary disease involving Pink1/Parkin-mediated mitophagy.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS The instruments used in this experiment mainly included the nose and mouth exposure inhalation tower, the Melton Inhalogic Nies Nose-only inhalation exposure system (China), whole body plethysmography (WBP, DSI), a microplate reader (Molecular Devices, SpectraMax i3x), a high-speed refrigerated centrifuge (Sigma, 3-18KS) and a smoke generator (Suzhou Alos Environmental Generator Co., Ltd., SPG-C). .. Additional materials included Hongyun cigarettes (Hebei Tobacco Industry Co., Ltd., bar code: 6901028250542), Pentobarbital Sodium (MERCK, P11011), bacterial lipopolysaccharide (LPS) dry powder (Sigma, 120M4030V), dexamethasone sodium phosphate injection (Guizhou Tiandi Pharmaceutical Co., Ltd., 19110802A), TRQ (inhalation solution, 6.4 mg/ml baicalin, Shanghai Kaibao Pharmaceutical Co., Ltd., 1906303X), Mitophagy Detection Kit (Dojindo Laboratories, MD01), MitoTracker Red CMX Ros (Invitrogen, M46752). ..

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